How are crimes solved by PCR?
The scene of the crime The body of a woman is found behind an abandoned warehouse on the outskirts of town. Forensic experts carry out a technical examination of the scene and suggest strangulation as...
View ArticleGet Out of Western Blot Hell: An Intro to Mass Spectrometry.
Mass Spectrometer: A Magical Way to ID Your Proteins After you finish immunoprecipitating a protein or purifying a subcellular compartment, you need to identify what proteins you purified. You could...
View ArticleThe different phases of PCR and why they are important
PCR (Polymerase Chain Reaction) is a biochemical technique developed by Kary Mullis in 1983 that is used to create large quantities of a sequence of DNA. Since this method of mass-producing DNA was...
View ArticleOntraport Test
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View ArticleDo-it-Yourself PCR
Currently Open Source principles are offering interesting tools for doing molecular biology at an incredibly low cost. One interesting example is OpenPCR (www.openPCR.org) a project developed in order...
View ArticleThe Devil is in the Details: How to Setup a PCR Laboratory
There is a right way and a wrong way to set up a PCR laboratory. Because of PCR’s tremendous ability to amplify small quantities of DNA/RNA template, even the smallest of template contamination can...
View ArticleThe A-Z of PCR variants
The wide range of applications of PCR has led to an ever-growing list of variants of the technique. While some are optimizations to suit specific requirements and are very similar to basic PCR, others...
View ArticleMIQE Guidelines for Digital PCR
MIQE what’s that? When writing dPCR materials and methods for a paper have you ever pondered what information you should include? This is where the MIQE guidelines will really help. Guidelines for...
View ArticleThe History of PCR
As with some of the greatest discoveries in science, from penicillin to microwave ovens and play-doh, PCR was discovered serendipitously. Thanks to the work of many scientists, including Watson and...
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